Pierre Alusta
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Research Scientist
Also affiliated: United States Food and Drug Administration (2015–2025); Central Arkansas Veterans Healthcare System (2011)
Staff Researcher
Research Areas
Biomedical Subjects
Links
Biography and Research Information
OverviewAI-generated summary
Pierre Alusta's research focuses on the detection and enumeration of microorganisms, particularly in environmental and pharmaceutical contexts. He has investigated methods for identifying specific bacterial species, such as the Burkholderia cepacia complex, using flow cytometry and fluorescence-labeled oligonucleotide probes. His work also includes developing flow cytometric assays to assess the viability and persistence of bacteria like Salmonella enterica in various conditions, including nuclease-free water and in the presence of antimicrobial agents like thymol. Alusta has also explored metagenomic approaches coupled with enrichment techniques for detecting microorganisms in potable groundwater and in non-sterilized pharmaceutical products. His research extends to the direct detection and identification of viruses in saliva using mass spectrometry. Alusta has published 28 papers, with 243 citations, and maintains an h-index of 9. He frequently collaborates with researchers at the National Center for Toxicological Research, including Soumana Daddy Gaoh, Dan A. Buzatu, Youngbeom Ahn, and Angel Paredes.
Metrics
- h-index: 9
- Publications: 28
- Citations: 246
Selected Publications
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Examining the viability of five Salmonella enterica subsp. enterica in thymol at 4°C and 25°C using flow cytometry (2025)
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A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples (2025)
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Direct detection and identification of viruses in saliva using a SpecID ionization modified mass spectrometer (2025)
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A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms (2024)
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A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products (2024)
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A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C (2024)
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Direct Detection and Identification of Viruses in Saliva Using a SpecID™ Ionization Modified Mass Spectrometer (2022)
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Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe (2022)
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A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of <i>Burkholderia cepacia</i> complex in nuclease-free water and antiseptics (2020)
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Estimating Bacterial Concentrations in Fibrous Substrates Through a Combination of Scanning Electron Microscopy and ImageJ (2019)
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Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique (2017)
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Feature selection from mass spectra of bacteria for serotyping Salmonella (2016)
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Instrumental improvements and sample preparations that enable reproducible, reliable acquisition of mass spectra from whole bacterial cells (2015)
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Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach (2015)
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Thymol treatment of bacteria prior to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analysis aids in identifying certain bacteria at the subspecies level (2014)
Collaboration Network
Top Collaborators
- Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
Showing 5 of 6 shared publications
- Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- Direct Detection and Identification of Viruses in Saliva Using a SpecID™ Ionization Modified Mass Spectrometer
- Direct detection and identification of viruses in saliva using a SpecID ionization modified mass spectrometer
- Examining the viability of five Salmonella enterica subsp. enterica in thymol at 4°C and 25°C using flow cytometry
- Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- Examining the viability of five Salmonella enterica subsp. enterica in thymol at 4°C and 25°C using flow cytometry
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- Direct detection and identification of viruses in saliva using a SpecID ionization modified mass spectrometer
- Examining the viability of five Salmonella enterica subsp. enterica in thymol at 4°C and 25°C using flow cytometry
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
- Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- Direct Detection and Identification of Viruses in Saliva Using a SpecID™ Ionization Modified Mass Spectrometer
- Direct detection and identification of viruses in saliva using a SpecID ionization modified mass spectrometer
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- Examining the viability of five Salmonella enterica subsp. enterica in thymol at 4°C and 25°C using flow cytometry
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
- Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
- Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
- Direct Detection and Identification of Viruses in Saliva Using a SpecID™ Ionization Modified Mass Spectrometer
- Direct Detection and Identification of Viruses in Saliva Using a SpecID™ Ionization Modified Mass Spectrometer
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