Pierre Alusta
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Research Scientist
Also affiliated: United States Food and Drug Administration (2010–2025); University of Arkansas System (2006); Central Arkansas Veterans Healthcare System (2011)
Research Areas
Biomedical Subjects
Links
Biography and Research Information
OverviewAI-generated summary
Pierre Alusta's research focuses on the detection and enumeration of microorganisms, particularly in environmental and pharmaceutical contexts. He has investigated methods for identifying specific bacterial species, such as the Burkholderia cepacia complex, using flow cytometry and fluorescence-labeled oligonucleotide probes. His work also includes developing flow cytometric assays to assess the viability and persistence of bacteria like Salmonella enterica in various conditions, including nuclease-free water and in the presence of antimicrobial agents like thymol. Alusta has also explored metagenomic approaches coupled with enrichment techniques for detecting microorganisms in potable groundwater and in non-sterilized pharmaceutical products. His research extends to the direct detection and identification of viruses in saliva using mass spectrometry. Alusta has published 28 papers, with 243 citations, and maintains an h-index of 9. He frequently collaborates with researchers at the National Center for Toxicological Research, including Soumana Daddy Gaoh, Dan A. Buzatu, Youngbeom Ahn, and Angel Paredes.
Metrics
- h-index: 9
- Publications: 28
- Citations: 249
Positions
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Research Scientist 2008–presentNational Center for Toxicological Research Div. of Systems Biology ORCID
Selected Publications
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Examining the viability of five Salmonella enterica subsp. enterica in thymol at 4°C and 25°C using flow cytometry (2025)
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A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples (2025)
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Direct detection and identification of viruses in saliva using a SpecID ionization modified mass spectrometer (2025)
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A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms (2024)
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A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products (2024)
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A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C (2024)
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Direct Detection and Identification of Viruses in Saliva Using a SpecID™ Ionization Modified Mass Spectrometer (2022)
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Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe (2022)
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A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics (2020)
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Estimating Bacterial Concentrations in Fibrous Substrates Through a Combination of Scanning Electron Microscopy and ImageJ (2019)
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Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique (2017)
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Feature selection from mass spectra of bacteria for serotyping Salmonella (2016)
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Instrumental improvements and sample preparations that enable reproducible, reliable acquisition of mass spectra from whole bacterial cells (2015)
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Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach (2015)
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Thymol treatment of bacteria prior to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analysis aids in identifying certain bacteria at the subspecies level (2014)
Collaboration Network
Top Collaborators
- A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics
- Thymol treatment of bacteria prior to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analysis aids in identifying certain bacteria at the subspecies level
- Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach
- Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique
- Improving proton MR spectroscopy of brain tissue for noninvasive diagnostics
Showing 5 of 13 shared publications
- Scanning Electron and Atomic Force Microscopy to Study Plasma Torch Effects on B. cereus Spores
- Killing of Bacillus spores is mediated by nitric oxide and nitric oxide synthase during glycoconjugate–enhanced phagocytosis
- Glycoconjugates enhanced the intracellular killing of Bacillus spores, increasing macrophage viability and activation
- Polymeric glycoconjugates protect and activate macrophages to promote killing of Bacillus cereus spores during phagocytosis
- Instrumental improvements and sample preparations that enable reproducible, reliable acquisition of mass spectra from whole bacterial cells
Showing 5 of 12 shared publications
- A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics
- Thymol treatment of bacteria prior to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analysis aids in identifying certain bacteria at the subspecies level
- Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach
- Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique
- Instrumental improvements and sample preparations that enable reproducible, reliable acquisition of mass spectra from whole bacterial cells
Showing 5 of 7 shared publications
- Thymol treatment of bacteria prior to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analysis aids in identifying certain bacteria at the subspecies level
- Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach
- Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique
- Improving proton MR spectroscopy of brain tissue for noninvasive diagnostics
- Instrumental improvements and sample preparations that enable reproducible, reliable acquisition of mass spectra from whole bacterial cells
Showing 5 of 7 shared publications
- Killing of Bacillus spores is mediated by nitric oxide and nitric oxide synthase during glycoconjugate–enhanced phagocytosis
- Glycoconjugates enhanced the intracellular killing of Bacillus spores, increasing macrophage viability and activation
- Polymeric glycoconjugates protect and activate macrophages to promote killing of Bacillus cereus spores during phagocytosis
- Glycoconjugates prevent B. anthracis toxin-induced cell death through binding while activating macrophages
- P7. Nitric oxide involved in macrophage activation and neutralization of Bacillus anthracis toxins during glycoconjugate treatment
Showing 5 of 6 shared publications
- A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics
- Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
Showing 5 of 6 shared publications
- Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
Showing 5 of 6 shared publications
- Killing of Bacillus spores is mediated by nitric oxide and nitric oxide synthase during glycoconjugate–enhanced phagocytosis
- Glycoconjugates prevent B. anthracis toxin-induced cell death through binding while activating macrophages
- P7. Nitric oxide involved in macrophage activation and neutralization of Bacillus anthracis toxins during glycoconjugate treatment
- Neutralization of B. anthracis toxins during ex vivo phagocytosis
- A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
- A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
- Thymol treatment of bacteria prior to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric analysis aids in identifying certain bacteria at the subspecies level
- Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach
- Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique
- A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
- A flow cytometric assay to detect viability and persistence of Salmonella enterica subsp. enterica serotypes in nuclease-free water at 4 and 25°C
- Direct detection and identification of viruses in saliva using a SpecID ionization modified mass spectrometer
- Examining the viability of five Salmonella enterica subsp. enterica in thymol at 4°C and 25°C using flow cytometry
- A metagenomic analysis coupled with oligotrophic enrichment approach for detecting specified microorganisms in potable groundwater samples
- A Comparative Metagenomic Analysis of Specified Microorganisms in Groundwater for Non-Sterilized Pharmaceutical Products
- A Metagenomic Analysis with Oligotrophic Enrichment Approach for Detecting Specified Microorganisms
- Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach
- Rapid Flow Cytometry Detection of a Single Viable Escherichia coli O157:H7 Cell in Raw Spinach Using a Simplified Sample Preparation Technique
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