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Listed
Presence
Current · Arkansas
Last published
2026
Sources
OpenAlex · ORCID
Refreshed
2026-10-05
H
Hari B. Krishnan
Research Molecular Biologist
55 h-index
284 pubs
9,221 cited
Research Areas
Biomedical Subjects
Links
Biography and Research Information
OverviewAI-generated summary
Hari B. Krishnan's research focuses on molecular biology, with a particular emphasis on plant proteins, genetics, and their applications. His work investigates methods to enhance the nutritional content of soybeans, including increasing protein and sulfur amino acid levels. He has explored the utilization of byproducts from tofu processing, such as wastewater, as a source for bioactive peptides like lunasin. Krishnan has also studied the ice recrystallization inhibition activity of soy protein hydrolysates and the potential of other plant-based protein resources, such as Adenanthera pavonina, examining their seed protein composition and the localization of trypsin inhibitors.
Further research by Krishnan delves into the genetic mechanisms of plant development, including the hypermethylation of miRNA genes during nodule development in soybeans. He has also investigated protein inhibitors, such as Bowman-Birk inhibitors, and their activities, employing techniques like CRISPR-Cas9 to generate mutants and study their effects on trypsin and chymotrypsin inhibitor levels. His work has also addressed practical applications, such as developing cost-effective procedures for measuring trypsin inhibitor activity in soy products and exploring the anti-inflammatory properties of lunasin concentrates in models of ulcerative colitis.
Metrics
- h-index: 55
- Publications: 284
- Citations: 9,221
Positions
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Research Molecular Biologist 1999–presentUSDA Agricultural Research Service Plant Genetics Research Unit ORCID
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Research Molecular BiologistArkansas Agricultural Experiment Station ORCID
Selected Publications
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Single-nucleus RNA-sequencing reveals the cellular programs driving nematode-induced giant cell formation in tomato (2025)
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13. Evaluation of soybean-derived trypsin inhibitor proteins on gastric emptying, luminal pH, and endogenous enzyme activity in late-stage nursery pigs (2025)
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Increasing dietary soybean-derived trypsin inhibitor protein compromises nursery pig performance, nitrogen digestibility, and retention (2025)
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Increasing trypsin inhibitor concentrations in growing pig diets compromises growth performance and protein digestibility (2025)
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Seed-Specific Silencing of Abundantly Expressed Soybean Bowman–Birk Protease Inhibitor Genes by RNAi Lowers Trypsin and Chymotrypsin Inhibitor Activities and Enhances Protein Digestibility (2025)
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Increasing dietary soybean trypsin inhibitor protein attenuates nursery pig performance (2025)
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Identification of an ice recrystallization inhibiting albumin protein from wheat flour and insights from molecular dynamics simulation (2025)
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Isolation and characterization of ice recrystallization inhibitory molecules from black soldier fly larvae (2025)
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Genomic and biochemical comparison of allelic triple‐mutant lines derived from conventional breeding and multiplex gene editing (2025)
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Enzymatic hydrolysis altered the physicochemical and immunogenic profile of protein-based ingredients derived from industrial hempseed (Cannabis sativa L.) (2025)
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14 The impact of soybean derived trypsin inhibitor concentrations on growth performance, health, and variability of nursery pigs (2025)
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Supplementation of multienzyme with acidifier alleviates the antinutritional effects of a high soybean meal diet for nursery pigs (2025)
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High-Resolution Mass Spectrometry Approach for Proteomic and Metabolomic Analyses of High-Protein Soybean Seeds (2025)
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Effects of feeding expeller-extruded high oleic soybeans in broiler diets on growth performance, blood profile, and meat quality (2025)
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Laboratory assays do not consistently indicate under processing of soybean meal that negatively impacts broiler performance (2025)
Collaboration Network
Top Collaborators
Won‐Seok Kim
University of Missouri (US)
42 shared publications
- All Three Subunits of Soybean β-Conglycinin Are Potential Food Allergens
- Extracellular Proteins Involved in Soybean Cultivar-Specific Nodulation Are Associated with Pilus-Like Surface Appendages and Exported by a Type III Protein Secretion System in Sinorhizobium fredii USDA257
- Transgenic soybean plants overexpressing O-acetylserine sulfhydrylase accumulate enhanced levels of cysteine and Bowman–Birk protease inhibitor in seeds
- Identification of a New Soybean Kunitz Trypsin Inhibitor Mutation and Its Effect on Bowman−Birk Protease Inhibitor Content in Soybean Seed
- Expression of an 11 kDa methionine‐rich delta‐zein in transgenic soybean results in the formation of two types of novel protein bodies in transitional cells situated between the vascular tissue and storage parenchyma cells
Showing 5 of 42 shared publications
Steven G. Pueppke
Plant (United States) (US)
33 shared publications
- Nod factors of Rhizobium are a key to the legume door
- Molecular cloning and characterization of a sym plasmid locus that regulates cultivar‐specific nodulation of soybean by Rhizobium fredii USDA257
- Extracellular Proteins Involved in Soybean Cultivar-Specific Nodulation Are Associated with Pilus-Like Surface Appendages and Exported by a Type III Protein Secretion System in Sinorhizobium fredii USDA257
- Characterization and localization of rice (Oryza sativa L.) seed globulins
- Structures of Nodulation Factors from the Nitrogen-Fixing Soybean Symbiont Rhizobium fredii USDA257
Showing 5 of 33 shared publications
Savithiry Natarajan
Beltsville Agricultural Research Center (US)
20 shared publications
- A rapid and simple procedure for the depletion of abundant storage proteins from legume seeds to advance proteome analysis: A case study using Glycine max
- Effect of Six Decades of Selective Breeding on Soybean Protein Composition and Quality: A Biochemical and Molecular Analysis
- A rapid method for depletion of Rubisco from soybean (Glycine max) leaf for proteomic analysis of lower abundance proteins
- An efficient extraction method to enhance analysis of low abundant proteins from soybean seed
- Identification of Glycinin and β-Conglycinin Subunits that Contribute to the Increased Protein Content of High-Protein Soybean Lines
Showing 5 of 20 shared publications
Joseph M. Jez
Washington University in St. Louis (US)
16 shared publications
- Review: The promise and limits for enhancing sulfur-containing amino acid content of soybean seed
- Assembly of the Cysteine Synthase Complex and the Regulatory Role of Protein-Protein Interactions
- Transgenic soybean plants overexpressing O-acetylserine sulfhydrylase accumulate enhanced levels of cysteine and Bowman–Birk protease inhibitor in seeds
- From sulfur to homoglutathione: thiol metabolism in soybean
- Soybean ATP sulfurylase, a homodimeric enzyme involved in sulfur assimilation, is abundantly expressed in roots and induced by cold treatment
Showing 5 of 16 shared publications
Nathan W. Oehrle
Agricultural Research Service (US)
15 shared publications
- A rapid and simple procedure for the depletion of abundant storage proteins from legume seeds to advance proteome analysis: A case study using Glycine max
- Effect of Heat Stress on Seed Protein Composition and Ultrastructure of Protein Storage Vacuoles in the Cotyledonary Parenchyma Cells of Soybean Genotypes That Are Either Tolerant or Sensitive to Elevated Temperatures
- Proteomic Analysis of Pigeonpea (Cajanus cajan) Seeds Reveals the Accumulation of Numerous Stress-Related Proteins
- Soybean Mutants Lacking Abundant Seed Storage Proteins Are Impaired in Mobilization of Storage Reserves and Germination
- Development of soybean experimental lines with enhanced protein and sulfur amino acid content
Showing 5 of 15 shared publications
Jason D. Gillman
Agricultural Research Service (US)
12 shared publications
- Impact of heat stress during seed development on soybean seed metabolome
- Identification of a New Soybean Kunitz Trypsin Inhibitor Mutation and Its Effect on Bowman−Birk Protease Inhibitor Content in Soybean Seed
- Effect of Heat Stress on Seed Protein Composition and Ultrastructure of Protein Storage Vacuoles in the Cotyledonary Parenchyma Cells of Soybean Genotypes That Are Either Tolerant or Sensitive to Elevated Temperatures
- Genomewide association study of ionomic traits on diverse soybean populations from germplasm collections
- Transcriptomic Profiling of Lathyrus sativus L. Metabolism of β-ODAP, a Neuroexcitatory Amino Acid Associated with Neurodegenerative Lower Limb Paralysis
Showing 5 of 12 shared publications
Bo Song
Harbin Normal University (CN)
12 shared publications
- Soybean Mutants Lacking Abundant Seed Storage Proteins Are Impaired in Mobilization of Storage Reserves and Germination
- Development of soybean experimental lines with enhanced protein and sulfur amino acid content
- Transcriptome Profile of Near-Isogenic Soybean Lines for β-Conglycinin α-Subunit Deficiency during Seed Maturation
- Development and Characterization of a Soybean Experimental Line Lacking the α′ Subunit of β-Conglycinin and G1, G2, and G4 Glycinin
- Identification and Characterization of β-Lathyrin, an Abundant Glycoprotein of Grass Pea ( Lathyrus sativus L.), as a Potential Allergen
Showing 5 of 12 shared publications
Vermont P. Día
University of Tennessee at Knoxville (US)
12 shared publications
- BG-4, a novel anticancer peptide from bitter gourd (Momordica charantia), promotes apoptosis in human colon cancer cells
- BG-4, a novel bioactive peptide from momordica charantia, inhibits lipopolysaccharide-induced inflammation in THP-1 human macrophages
- Kunitz trypsin inhibitor in addition to Bowman-Birk inhibitor influence stability of lunasin against pepsin-pancreatin hydrolysis
- Utilization of tofu processing wastewater as a source of the bioactive peptide lunasin
- Ice Recrystallization Inhibition Activity of Soy Protein Hydrolysates
Showing 5 of 12 shared publications
Shanshan Liu
Northeast Agricultural University (CN)
9 shared publications
- Soybean Mutants Lacking Abundant Seed Storage Proteins Are Impaired in Mobilization of Storage Reserves and Germination
- Transcriptome Profile of Near-Isogenic Soybean Lines for β-Conglycinin α-Subunit Deficiency during Seed Maturation
- Development and Characterization of a Soybean Experimental Line Lacking the α′ Subunit of β-Conglycinin and G1, G2, and G4 Glycinin
- Identification and Characterization of β-Lathyrin, an Abundant Glycoprotein of Grass Pea ( Lathyrus sativus L.), as a Potential Allergen
- Characterization of Seed Storage Proteins of Several Perennial Glycine Species
Showing 5 of 9 shared publications
Sun‐Hyung Kim
University of Missouri (US)
9 shared publications
- A fast and cost-effective procedure for reliable measurement of trypsin inhibitor activity in soy and soy products
- Bowman–Birk Inhibitor Mutants of Soybean Generated by CRISPR-Cas9 Reveal Drastic Reductions in Trypsin and Chymotrypsin Inhibitor Activities
- Adenanthera pavonina, a potential plant-based protein resource: Seed protein composition and immunohistochemical localization of trypsin inhibitors
- Seed-Specific Silencing of Abundantly Expressed Soybean Bowman–Birk Protease Inhibitor Genes by RNAi Lowers Trypsin and Chymotrypsin Inhibitor Activities and Enhances Protein Digestibility
- Differential symbiotic compatibilities between rhizobium strains and cultivated and wild soybeans revealed by anatomical and transcriptome analyses
Showing 5 of 9 shared publications
Thomas W. Okita
Washington State University (US)
8 shared publications
- Immunochemical studies on the role of the Golgi complex in protein-body formation in rice seeds
- Structure and Expression of the Rice Glutelin Multigene Family
- Structural Relationship among the Rice Glutelin Polypeptides
- Wheat Invertases
- Formation of wheat protein bodies: Involvement of the Golgi apparatus in gliadin transport
Showing 5 of 8 shared publications
Ammulu Hari Krishnan
Agricultural Research Service (US)
8 shared publications
- A Functional myo -Inositol Dehydrogenase Gene Is Required for Efficient Nitrogen Fixation and Competitiveness of Sinorhizobium fredii USDA191 To Nodulate Soybean ( Glycine max [L.] Merr.)
- Positional Effect on Protein and Oil Content and Composition of Soybeans
- Nitrogen Lowers the Sulfur Amino Acid Content of Soybean (Glycine max [L.] Merr.) by Regulating the Accumulation of Bowman−Birk Protease Inhibitor
- Seed storage protein composition of non-nodulating soybean (Glycine max (L.) Merr.) and its influence on protein quality
- Rhizobium etli USDA9032 Engineered To Produce a Phenazine Antibiotic Inhibits the Growth of Fungal Pathogens but Is Impaired in Symbiotic Performance
Showing 5 of 8 shared publications
Kil Yong Kim
Chonnam National University (KR)
8 shared publications
- Rahnella aquatilis, a bacterium isolated from soybean rhizosphere, can solubilize hydroxyapatite1
- Extracellular Proteins Involved in Soybean Cultivar-Specific Nodulation Are Associated with Pilus-Like Surface Appendages and Exported by a Type III Protein Secretion System in Sinorhizobium fredii USDA257
- Expression of genes fromRahnella aquatilisthat are necessary for mineral phosphate solubilization inEscherichia coli
- Effect of chitin compost and broth on biological control of Meloidogyne incognita on tomato (Lycopersicon esculentum Mill.)
- Control of Late Blight (Phytophthora capsici ) in Pepper Plant with a Compost Containing Multitude of Chitinase-producing Bacteria
Showing 5 of 8 shared publications
Nazrul Islam
Genomics (United Kingdom) (GB)
7 shared publications
- Quantitative Proteomic Analysis of Low Linolenic Acid Transgenic Soybean Reveals Perturbations of Fatty Acid Metabolic Pathways
- Proteomic Profiling of Fast Neutron-Induced Soybean Mutant Unveiled Pathways Associated with Increased Seed Protein Content
- Quantitative proteomic analyses reveal the dynamics of protein and amino acid accumulation during soybean seed development
- Proteomic Comparison of Three Extraction Methods Reveals the Abundance of Protease Inhibitors in the Seeds of Grass Pea, a Unique Orphan Legume
- Protein profiling of fast neutron soybean mutant seeds reveals differential accumulation of seed and iron storage proteins
Showing 5 of 7 shared publications
Nicholas K Gabler
Iowa State University (US)
7 shared publications
- 137 Impact of dietary soybean-mediated trypsin inhibitor concentrations on nursery pig performance
- 246 The effect of trypsin inhibitor unit concentrations from raw soybeans in grower pig performance and protein digestibility
- Increasing dietary soybean-derived trypsin inhibitor protein compromises nursery pig performance, nitrogen digestibility, and retention
- 14 The impact of soybean derived trypsin inhibitor concentrations on growth performance, health, and variability of nursery pigs
- Increasing dietary soybean trypsin inhibitor protein attenuates nursery pig performance
Showing 5 of 7 shared publications
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