Vladimir Lupashin
Professor
University of Arkansas for Medical Sciences
faculty
Physiology & Cell Biology, College of Medicine
Research Areas
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Biography and Research Information
OverviewAI-generated summary
Vladimir Lupashin's research laboratory investigates the molecular mechanisms governing the formation and maintenance of intracellular membrane-bounded compartments. Intracellular membrane trafficking is fundamental to essential cellular functions such as protein secretion, post-translational modifications, cell signaling, cell polarization, and cell maintenance. Disruptions in this process are implicated in various human diseases, including cancer, diabetes mellitus, Alzheimer's disease, cystic fibrosis, Hermansky-Pudlak syndrome, and Congenital Disorders of Glycosylation.
The laboratory utilizes both yeast and mammalian tissue culture cell models to explore the basic mechanisms of intracellular vesicular trafficking. This work has contributed to the discovery of novel vesicle tethering factors. Lupashin has published over 60 original papers in high-profile journals, including the Journal of Cell Biology, PNAS, Science, the Journal of Neuroscience, Molecular Biology of the Cell, and Nature Communications. His research has been consistently supported by grants from the NSF and NIH. He has received funding for the "Characterization of mammalian COG complex-interacting Golgi trafficking machinery" from the NIH/National Institute of General Medical Sciences for $409,543.
Lupashin's scholarship is recognized by a high-impact researcher designation, evidenced by an h-index of 41 and over 5,130 citations across 125 publications. He collaborates with researchers at the University of Arkansas for Medical Sciences, including Irina D. Pokrovskaya, Amrita Khakurel, Farhana Taher Sumya, and Zinia D’Souza, with whom he has co-authored multiple publications.
Research Overview
Our laboratory is interested in understanding the molecular mechanisms responsible for the generation and maintenance of intra-cellular membrane-bounded compartments. In all eukaryotic cells intracellular membrane trafficking is critical for a range of important cellular functions including protein secretion, post-translational modifications, cell signalling, cell polarization, and cell maintenance. Defects in membrane trafficking can underline, or even exacerbate, a number of human diseases including cancer, diabetes mellitus, Alzheimer’s, cystic fibrosis, Hermansky-Pudlak syndrome and Congenital Disorders of Glycosylation. Our research directed towards the understanding of the basic mechanisms of intracellular vesicular trafficking using both yeast and mammalian tissue culture cell model systems. Our lab played a principal role in the discovery of a novel vesicle tethering factors, published more than 60 original papers in high-profile journals, including Journal of Cell Biology, PNAS, Science, Journal of Neuroscience, Molecular Biology of Cell and Nature Communications. My current research has been continuously supported by grants from both NSF and NIH. We have pioneered the functional analysis of the Conserved Oligomeric Golgi (COG), an evolutionarily conserved complex of eight gene products, each of which is critical for the membrane trafficking and protein modifications in the Golgi apparatus. The COG complex interacts with core fusion machinery components including SNAREs, SM proteins, Rabs, coiled-coil tethers and COPI coat to organize specific docking and fusion of transport intermediates with their acceptor membrane. By using state of the art biochemical, genetic and microscopy approaches (including mass-spectrometry, electron and super-resolution microscopy, CRISPR directed gene editing techniques) we would like to determine how the key components of intracellular membrane trafficking machinery work together to direct efficient protein trafficking in human cells in health and disease.
Metrics
- h-index: 41
- Publications: 125
- Citations: 5,130
Selected Publications
- Deep proteomic profiling of the intra-Golgi trafficking intermediates (2025) DOI
- Acute <scp>GARP</scp> Depletion Disrupts Vesicle Transport, Leading to Severe Defects in Sorting, Secretion and <i>O</i> ‐Glycosylation (2025) DOI
- Comprehensive Proteomic Characterization of the Intra-Golgi Trafficking Intermediates (2024) DOI
- Acute GARP depletion disrupts vesicle transport, leading to severe defects in sorting, secretion, and O-glycosylation (2024) DOI
- Essential role of the conserved oligomeric Golgi complex in <i>Toxoplasma gondii</i> (2023) DOI
- Biallelic missense variants in <scp><i>COG3</i></scp> cause a congenital disorder of glycosylation with impairment of retrograde vesicular trafficking (2023) DOI
- Syntaxin‐5's flexibility in <scp>SNARE</scp> pairing supports Golgi functions (2023) DOI
- A Rab33b missense mouse model for Smith-McCort dysplasia shows bone resorption defects and altered protein glycosylation (2023) DOI
- Essential role of the Conserved Oligomeric Golgi complex in <i>Toxoplasma gondii</i> (2023) DOI
- Faculty Opinions recommendation of The K/HDEL receptor does not recycle but instead acts as a Golgi-gatekeeper. (2023) DOI
- Insights into the regulation of cellular Mn2+ homeostasis via TMEM165 (2023) DOI
- Role of GARP Vesicle Tethering Complex in Golgi Physiology (2023) DOI
- Correction to: Golgi (2023) DOI
- GARP dysfunction results in COPI displacement, depletion of Golgi v-SNAREs and calcium homeostasis proteins (2022) DOI
- Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System (2022) DOI
Federal Grants 1 $409,543 total
Characterization of mammalian COG complex-interacting Golgi trafficking machinery
Grants & Funding
- Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub NIH/Nat. Inst. of Allergy & Infectious Diseases - Pass Through: Washington State University Principal Investigator
- COM-Intramural Award UAMS College of Medicine Principal Investigator
- Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub NIH/Nat. Inst. of Allergy & Infectious Diseases - Pass Through: Washington State University Principal Investigator
- Functional Analysis of the conserved oligomeric Golgi (COG) complex in yeast National Science Foundation Principal Investigator
- Structural and functional analysis of SEC 34 Protein complex National Science Foundation Principal Investigator
- Characterization of mammalian COG complex-interacting Golgi trafficking machinery NIH Principal Investigator
- Characterization of mammalian COG complex-interacting Golgi trafficking machinery NIH/Nat. Inst. of General Medical Sciences Principal Investigator
- Functional Analysis of the conserved oligomeric Golgi (COG) complex in yeast National Science Foundation Principal Investigator
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