Vladimir V. Lupashin
Professor
Also affiliated: Dartmouth College (1998); University of North Carolina at Chapel Hill (2020); Russian Academy of Sciences (1987); National Research Tomsk State University (2014); Princeton University (1996–1999); University of Arkansas Medical Center (2008–2020); G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms (1987–1992); University of California, Berkeley (1996)
Physiology & Cell Biology, College of Medicine
Research Areas
Biomedical Subjects
Biography and Research Information
OverviewAI-generated summary
The laboratory of Vladimir V. Lupashin, Professor in Physiology & Cell Biology at the University of Arkansas for Medical Sciences, investigates the molecular mechanisms underlying the generation and maintenance of intracellular membrane-bounded compartments. Intracellular membrane trafficking is fundamental to numerous cellular functions, including protein secretion, post-translational modifications, cell signaling, and cell maintenance. Disruptions in this process are implicated in various human diseases such as cancer, diabetes mellitus, Alzheimer’s disease, cystic fibrosis, Hermansky-Pudlak syndrome, and Congenital Disorders of Glycosylation.
Research efforts focus on understanding the basic mechanisms of intracellular vesicular trafficking, employing both yeast and mammalian tissue culture cell model systems. The lab has contributed to the discovery of novel vesicle tethering factors and has published over 60 original papers in high-profile journals. Current research is supported by continuous funding from the NSF and NIH. Dr. Lupashin's work has been recognized as high-impact, indicated by a high h-index of 42 and over 5,400 citations. He has served as PI on a $409,543 NIH grant focused on characterizing mammalian COG complex-interacting Golgi trafficking machinery.
Research Overview
Our laboratory is interested in understanding the molecular mechanisms responsible for the generation and maintenance of intra-cellular membrane-bounded compartments. In all eukaryotic cells intracellular membrane trafficking is critical for a range of important cellular functions including protein secretion, post-translational modifications, cell signalling, cell polarization, and cell maintenance. Defects in membrane trafficking can underline, or even exacerbate, a number of human diseases including cancer, diabetes mellitus, Alzheimer’s, cystic fibrosis, Hermansky-Pudlak syndrome and Congenital Disorders of Glycosylation. Our research directed towards the understanding of the basic mechanisms of intracellular vesicular trafficking using both yeast and mammalian tissue culture cell model systems. Our lab played a principal role in the discovery of a novel vesicle tethering factors, published more than 60 original papers in high-profile journals, including Journal of Cell Biology, PNAS, Science, Journal of Neuroscience, Molecular Biology of Cell and Nature Communications. My current research has been continuously supported by grants from both NSF and NIH. We have pioneered the functional analysis of the Conserved Oligomeric Golgi (COG), an evolutionarily conserved complex of eight gene products, each of which is critical for the membrane trafficking and protein modifications in the Golgi apparatus. The COG complex interacts with core fusion machinery components including SNAREs, SM proteins, Rabs, coiled-coil tethers and COPI coat to organize specific docking and fusion of transport intermediates with their acceptor membrane. By using state of the art biochemical, genetic and microscopy approaches (including mass-spectrometry, electron and super-resolution microscopy, CRISPR directed gene editing techniques) we would like to determine how the key components of intracellular membrane trafficking machinery work together to direct efficient protein trafficking in human cells in health and disease.
Metrics
- h-index: 42
- Publications: 127
- Citations: 5,526
Positions
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Professor 2012–presentUniversity of Arkansas for Medical Sciences Physiology & Cell Biology, College of Medicine Institutional directory
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Associate Professor 2006–2012University of Arkansas for Medical Sciences College of Medicine Physiology and Biophysics ORCID
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Assistant Professor 1998–2006University of Arkansas for Medical Sciences College of Medicine Physiology and Biophisics ORCID
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Research Associate 1995–1998Princeton University Molecular Biology ORCID
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Research Associate 1991–1994University of California Berkeley MCB ORCID
Selected Publications
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Golgi CATCHR complexes function as organizing hubs for vesicle tethering and fusion (2026)
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COG Complex in Golgi Trafficking and Glycosylation (2026)
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GARP Complex in Golgi Physiology (2026)
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Deep proteomic profiling of the intra-Golgi trafficking intermediates (2025)
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Acute GARP Depletion Disrupts Vesicle Transport, Leading to Severe Defects in Sorting, Secretion and O ‐Glycosylation (2025)
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Comprehensive Proteomic Characterization of the Intra-Golgi Trafficking Intermediates (2024)
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Acute GARP depletion disrupts vesicle transport, leading to severe defects in sorting, secretion, and O-glycosylation (2024)
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Essential role of the conserved oligomeric Golgi complex in Toxoplasma gondii (2023)
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Biallelic missense variants in COG3 cause a congenital disorder of glycosylation with impairment of retrograde vesicular trafficking (2023)
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Syntaxin‐5's flexibility in SNARE pairing supports Golgi functions (2023)
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A Rab33b missense mouse model for Smith-McCort dysplasia shows bone resorption defects and altered protein glycosylation (2023)
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Essential role of the Conserved Oligomeric Golgi complex in Toxoplasma gondii (2023)
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Faculty Opinions recommendation of The K/HDEL receptor does not recycle but instead acts as a Golgi-gatekeeper. (2023)
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Insights into the regulation of cellular Mn2+ homeostasis via TMEM165 (2023)
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Role of GARP Vesicle Tethering Complex in Golgi Physiology (2023)
Federal Grants 1 $409,543 total
Characterization of mammalian COG complex-interacting Golgi trafficking machinery
Grants & Funding
As listed on this researcher's institutional profile. Federal awards with verified records are shown above.
- Remodeling of intracellular membrane traffic by Brucella effectors- Washington State Sub NIH/Nat. Inst. of Allergy & Infectious Diseases via Washington State University Principal Investigator
- COM-Intramural Award UAMS College of Medicine Principal Investigator
- Functional Analysis of the conserved oligomeric Golgi (COG) complex in yeast National Science Foundation Principal Investigator
- Structural and functional analysis of SEC 34 Protein complex National Science Foundation Principal Investigator
- Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus Function NIH Co-Investigator
- Role of COG complex mediated vesicle tethering is localization of Golgi enzymes Mizutani Foundation for Glycoscience Principal Investigator
- Super-Resolution Light Microscope at University of Arkansas for Medical Sciences NIH Co-Investigator
- Modulation of host secretory trafficking by the Brucella effector BspB - Continuation - Continuation NIH/Nat. Inst. of General Medical Sciences via Washington State University Principal Investigator
Collaboration Network
Top Collaborators
- Conserved oligomeric Golgi complex specifically regulates the maintenance of Golgi glycosylation machinery
- COG complexes form spatial landmarks for distinct SNARE complexes
- The COG Complex, Rab6 and COPI Define a Novel Golgi Retrograde Trafficking Pathway that is Exploited by SubAB Toxin
- COG Complex Complexities: Detailed Characterization of a Complete Set of HEK293T Cells Lacking Individual COG Subunits
- COG6 Interacts with a Subset of the Golgi SNAREs and Is Important for the Golgi Complex Integrity
Showing 5 of 22 shared publications
- Conserved oligomeric Golgi complex specifically regulates the maintenance of Golgi glycosylation machinery
- COG complexes form spatial landmarks for distinct SNARE complexes
- Molecular Insights into Vesicle Tethering at the Golgi by the Conserved Oligomeric Golgi (COG) Complex and the Golgin TATA Element Modulatory Factor (TMF)
- The COG Complex, Rab6 and COPI Define a Novel Golgi Retrograde Trafficking Pathway that is Exploited by SubAB Toxin
- COG6 Interacts with a Subset of the Golgi SNAREs and Is Important for the Golgi Complex Integrity
Showing 5 of 18 shared publications
- Maintaining order: COG complex controls Golgi trafficking, processing, and sorting
- A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication
- The interactome of the copper transporter ATP7A belongs to a network of neurodevelopmental and neurodegeneration factors
- COG Complex Complexities: Detailed Characterization of a Complete Set of HEK293T Cells Lacking Individual COG Subunits
- More than just sugars: Conserved oligomeric Golgi complex deficiency causes glycosylation‐independent cellular defects
Showing 5 of 12 shared publications
- The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery
- Role of GARP Vesicle Tethering Complex in Golgi Physiology
- Getting Sugar Coating Right! The Role of the Golgi Trafficking Machinery in Glycosylation
- GARP dysfunction results in COPI displacement, depletion of Golgi v-SNAREs and calcium homeostasis proteins
- Generation and Analysis of hTERT-RPE1 VPS54 Knock-Out and Rescued Cell Lines
Showing 5 of 11 shared publications
- Galactose Supplementation in Patients With TMEM165-CDG Rescues the Glycosylation Defects
- Defects in the COG complex and COG-related trafficking regulators affect neuronal Golgi function
- Manganese-induced turnover of TMEM165
- COG lobe B sub-complex engages v-SNARE GS15 and functions via regulated interaction with lobe A sub-complex
- Cog5–Cog7 crystal structure reveals interactions essential for the function of a multisubunit tethering complex
Showing 5 of 10 shared publications
- Maintaining order: COG complex controls Golgi trafficking, processing, and sorting
- Golgi inCOGnito: From vesicle tethering to human disease
- Defects in COG-Mediated Golgi Trafficking Alter Endo-Lysosomal System in Human Cells
- Getting Sugar Coating Right! The Role of the Golgi Trafficking Machinery in Glycosylation
- Acute COG complex inactivation unveiled its immediate impact on Golgi and illuminated the nature of intra‐Golgi recycling vesicles
Showing 5 of 10 shared publications
- Getting Sugar Coating Right! The Role of the Golgi Trafficking Machinery in Glycosylation
- Development and Initial Characterization of Cellular Models for COG Complex-Related CDG-II Diseases
- Acute COG complex inactivation unveiled its immediate impact on Golgi and illuminated the nature of intra‐Golgi recycling vesicles
- Biallelic missense variants in COG3 cause a congenital disorder of glycosylation with impairment of retrograde vesicular trafficking
- Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System
Showing 5 of 10 shared publications
- The Golgi puppet master: COG complex at center stage of membrane trafficking interactions
- Conserved oligomeric Golgi complex specifically regulates the maintenance of Golgi glycosylation machinery
- COG complexes form spatial landmarks for distinct SNARE complexes
- The COG Complex, Rab6 and COPI Define a Novel Golgi Retrograde Trafficking Pathway that is Exploited by SubAB Toxin
- COG6 Interacts with a Subset of the Golgi SNAREs and Is Important for the Golgi Complex Integrity
Showing 5 of 9 shared publications
- Characterization of a mammalian Golgi-localized protein complex, COG, that is required for normal Golgi morphology and function
- The Golgi puppet master: COG complex at center stage of membrane trafficking interactions
- COG8 deficiency causes new congenital disorder of glycosylation type IIh
- COG complexes form spatial landmarks for distinct SNARE complexes
- Molecular Insights into Vesicle Tethering at the Golgi by the Conserved Oligomeric Golgi (COG) Complex and the Golgin TATA Element Modulatory Factor (TMF)
Showing 5 of 9 shared publications
- The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins
- Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability
- Identification of a Human Orthologue of Sec34p as a Component of the cis-Golgi Vesicle Tethering Machinery
- Essential role of the conserved oligomeric Golgi complex in Toxoplasma gondii
- Essential role of the Conserved Oligomeric Golgi complex in Toxoplasma gondii
- Role of the conserved oligomeric Golgi (COG) complex in protein glycosylation
- Conserved oligomeric Golgi complex specifically regulates the maintenance of Golgi glycosylation machinery
- The COG Complex, Rab6 and COPI Define a Novel Golgi Retrograde Trafficking Pathway that is Exploited by SubAB Toxin
- Structural basis for a human glycosylation disorder caused by mutation of the COG4 gene
- Mislocalization of large ARF-GEFs as a potential mechanism for BFA resistance in COG-deficient cells
- Quantitative Proteomic and Genetic Analyses of the Schizophrenia Susceptibility Factor Dysbindin Identify Novel Roles of the Biogenesis of Lysosome-Related Organelles Complex 1
- The interactome of the copper transporter ATP7A belongs to a network of neurodevelopmental and neurodegeneration factors
- Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content
- Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content
- Author response: The interactome of the copper transporter ATP7A belongs to a network of neurodevelopmental and neurodegeneration factors
- Quantitative Proteomic and Genetic Analyses of the Schizophrenia Susceptibility Factor Dysbindin Identify Novel Roles of the Biogenesis of Lysosome-Related Organelles Complex 1
- The interactome of the copper transporter ATP7A belongs to a network of neurodevelopmental and neurodegeneration factors
- Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content
- Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content
- Author response: The interactome of the copper transporter ATP7A belongs to a network of neurodevelopmental and neurodegeneration factors
- Galactose Supplementation in Patients With TMEM165-CDG Rescues the Glycosylation Defects
- Manganese-induced turnover of TMEM165
- Dissection of TMEM165 function in Golgi glycosylation and its Mn2+ sensitivity
- Novel role for the Golgi membrane protein TMEM165 in control of migration and invasion for breast carcinoma
- Insights into the regulation of cellular Mn2+ homeostasis via TMEM165
- Serotonin Transamidates Rab4 and Facilitates Its Binding to the C Terminus of Serotonin Transporter
- RETRACTED ARTICLE: Sepsis-induced elevation in plasma serotonin facilitates endothelial hyperpermeability
- The Cellular Distribution of Serotonin Transporter Is Impeded on Serotonin-Altered Vimentin Network
- Retraction Note: Sepsis-induced elevation in plasma serotonin facilitates endothelial hyperpermeability
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