M
Mark Manzano
Federal Grant PI
Asssistant Professor
faculty
Microbiology & Immunology, College of Medicine
15 h-index
32 pubs
1,321 cited
Research Areas
Links
Biography and Research Information
OverviewAI-generated summary
Mark Manzano, an Assistant Professor in Microbiology & Immunology at the University of Arkansas for Medical Sciences, investigates host-pathogen interactions and biological processes using functional genomics, particularly CRISPR screens. His laboratory's work focuses on Primary Effusion Lymphoma (PEL), an aggressive B cell cancer associated with the Kaposi’s sarcoma-associated herpesvirus (KSHV/HHV8). PEL cells depend on viral genes that alter host gene expression to promote tumor cell proliferation and survival. Manzano's research has identified over 200 host genes essential for PEL cell growth and survival, aiming to elucidate their specific functions within B cell lymphoma.
Utilizing CRISPR/Cas9, CRISPRi, and CRISPRa technologies, his lab conducts genome-wide screens to uncover functional genetic interactions and identify synthetic lethality and rescue pathways. This unbiased approach seeks to reveal novel therapeutic targets and understand the molecular mechanisms underlying KSHV latency and KSHV-transformed cell line dependence on specific host genes, such as MCL1. Manzano has secured significant federal funding for his research, including multiple NIH grants totaling over $1.3 million, focusing on KSHV pathogenesis, oncogenic roles of viral genes, and epigenetic regulation of viral latency.
Metrics
- h-index: 15
- Publications: 32
- Citations: 1,321
Selected Publications
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Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems (2025)
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Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment (2025)
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The Mitochondrial Ubiquitin Ligase MARCHF5 Cooperates with MCL1 to Inhibit Apoptosis in KSHV-Transformed Primary Effusion Lymphoma Cell Lines (2024)
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Cytotoxicity of Activator Expression in CRISPR-based Transcriptional Activation Systems (2024)
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Molecular Mechanisms of KSHV Latency Establishment and Maintenance (2024)
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Suppression of TRIP13 Induces Metabolic Changes and Potentiates Ferroptosis in Multiple Myeloma (2023)
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Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment (2023)
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CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent, TRAIL-R1-mediated cell death (2023)
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Expression Ratios of the Antiapoptotic BCL2 Family Members Dictate the Selective Addiction of Kaposi’s Sarcoma-Associated Herpesvirus-Transformed Primary Effusion Lymphoma Cell Lines to MCL1 (2022)
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Expression Ratios of the Anti-Apoptotic BCL2 Family Members Dictate the Selective Addiction of KSHV-Transformed Primary Effusion Lymphoma Cell Lines to MCL1 (2022)
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CRISPR Screens Identify Novel Regulators of cFLIP Dependency and Ligand-Independent, TRAIL-R1-Mediated Cell Death (2022)
Federal Grants 4 $1,340,840 total
NIH/National Institute of Dental and Craniofacial Research
Contact PI
Jul 2025 - Mar 2030
NIH/National Institute of Allergy and Infectious Diseases
Co-PI
Jun 2025 - May 2027
Lytic viral genes in the pathogenesis of oncogenic gammaherpesviruses
NIH/National Cancer Institute
Contact PI
Aug 2023 - Jul 2025
Role of a latent OriLyt RNA in KSHV latency in primary effusion lymphoma
NIH/National Cancer Institute
Contact PI
Sep 2020 - Aug 2023
Oncogenic Roles and Therapeutic Potential of MCL1 Addiction in Primary Effusion Lymphoma
Grants & Funding
- Epigenetic Regulation of KSHV Latency NIH/Nat. Inst. of Dental & Craniofacial Research Principal Investigator
- Epigenetic Regulation of KSHV Latency NIH/Nat. Inst. of Dental & Craniofacial Research Principal Investigator
- Role of a latent OriLyt RNA in KSHV latency in primary effusion lymphoma NIH/Nat. Cancer Institute Principal Investigator
- Role of a latent OriLyt RNA in KSHV latency in primary effusion lymphoma NIH/Nat. Cancer Institute Principal Investigator
- Epigenetic Regulation of KSHV Latency NIH/Nat. Inst. of Dental & Craniofacial Research Principal Investigator
- Epigenetic Regulation of KSHV Latency NIH/Nat. Inst. of Dental & Craniofacial Research Principal Investigator
- Oncogenic Roles and Therapeutic Potential of MCL1 Addiction in Primary Effusion Lymphoma NIH/Nat. Cancer Institute Principal Investigator
- Role of a latent OriLyt RNA in KSHV latency in primary effusion lymphoma NIH/Nat. Cancer Institute Principal Investigator
Collaboration Network
Top Collaborators
Eva Gottwein
Northwestern University (US)
4 shared publications
- CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent, TRAIL-R1-mediated cell death
- CRISPR Screens Identify Novel Regulators of cFLIP Dependency and Ligand-Independent, TRAIL-R1-Mediated Cell Death
- Cytotoxicity of Activator Expression in CRISPR-based Transcriptional Activation Systems
- Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems
Prasanth Viswanathan
Winthrop Rockefeller Foundation
4 shared publications
In database
- Expression Ratios of the Antiapoptotic BCL2 Family Members Dictate the Selective Addiction of Kaposi’s Sarcoma-Associated Herpesvirus-Transformed Primary Effusion Lymphoma Cell Lines to MCL1
- Expression Ratios of the Anti-Apoptotic BCL2 Family Members Dictate the Selective Addiction of KSHV-Transformed Primary Effusion Lymphoma Cell Lines to MCL1
- Suppression of TRIP13 Induces Metabolic Changes and Potentiates Ferroptosis in Multiple Myeloma
- The Mitochondrial Ubiquitin Ligase MARCHF5 Cooperates with MCL1 to Inhibit Apoptosis in KSHV-Transformed Primary Effusion Lymphoma Cell Lines
Daniel Dunham
Winthrop Rockefeller Foundation
3 shared publications
In database
- Expression Ratios of the Antiapoptotic BCL2 Family Members Dictate the Selective Addiction of Kaposi’s Sarcoma-Associated Herpesvirus-Transformed Primary Effusion Lymphoma Cell Lines to MCL1
- Expression Ratios of the Anti-Apoptotic BCL2 Family Members Dictate the Selective Addiction of KSHV-Transformed Primary Effusion Lymphoma Cell Lines to MCL1
- The Mitochondrial Ubiquitin Ligase MARCHF5 Cooperates with MCL1 to Inhibit Apoptosis in KSHV-Transformed Primary Effusion Lymphoma Cell Lines
Jackson Gill
Winthrop Rockefeller Foundation
3 shared publications
In database
- Expression Ratios of the Antiapoptotic BCL2 Family Members Dictate the Selective Addiction of Kaposi’s Sarcoma-Associated Herpesvirus-Transformed Primary Effusion Lymphoma Cell Lines to MCL1
- Expression Ratios of the Anti-Apoptotic BCL2 Family Members Dictate the Selective Addiction of KSHV-Transformed Primary Effusion Lymphoma Cell Lines to MCL1
- The Mitochondrial Ubiquitin Ligase MARCHF5 Cooperates with MCL1 to Inhibit Apoptosis in KSHV-Transformed Primary Effusion Lymphoma Cell Lines
Steven Murdock
University of Arkansas for Medical Sciences
3 shared publications
In database
- Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment
- Molecular Mechanisms of KSHV Latency Establishment and Maintenance
- Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment
J. Craig Forrest
University of Arkansas for Medical Sciences
3 shared publications
In database
- Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment
- Molecular Mechanisms of KSHV Latency Establishment and Maintenance
- Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment
Neil Kuehnle
Northwestern University (US)
2 shared publications
- CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent, TRAIL-R1-mediated cell death
- CRISPR Screens Identify Novel Regulators of cFLIP Dependency and Ligand-Independent, TRAIL-R1-Mediated Cell Death
Scout Mask Osborne
Northwestern University (US)
2 shared publications
- CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent, TRAIL-R1-mediated cell death
- CRISPR Screens Identify Novel Regulators of cFLIP Dependency and Ligand-Independent, TRAIL-R1-Mediated Cell Death
Ziyan Liang
Northwestern University (US)
2 shared publications
- CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent, TRAIL-R1-mediated cell death
- CRISPR Screens Identify Novel Regulators of cFLIP Dependency and Ligand-Independent, TRAIL-R1-Mediated Cell Death
Shana M. Owens
University of Arkansas for Medical Sciences
2 shared publications
In database
- Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment
- Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment
Jeffrey M. Sifford
University of Arkansas for Medical Sciences
2 shared publications
In database
- Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment
- Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment
Eduardo Salinas
University of Arkansas for Medical Sciences
2 shared publications
In database
- Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment
- Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment
Debopam Ghosh
University of Arkansas for Medical Sciences
2 shared publications
In database
- Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment
- Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment
Jason S. Stumhofer
University of Arkansas for Medical Sciences
2 shared publications
In database
- Intrinsic p53 activation restricts gammaherpesvirus driven germinal center B cell expansion during latency establishment
- Intrinsic p53 Activation Restricts Gammaherpesvirus-Driven Germinal Center B Cell Expansion during Latency Establishment
Aakaanksha Maddineni
Northwestern University (US)
2 shared publications
- Cytotoxicity of Activator Expression in CRISPR-based Transcriptional Activation Systems
- Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems
Similar Researchers
Based on overlapping research topics
Daniel Dunham
University of Arkansas for Medical Sciences
Herpesvirus Infections and Treatments
Lymphoma Diagnosis and Treatment
Cancer-related molecular mechanisms research
Prasanth Viswanathan
University of Arkansas for Medical Sciences
Herpesvirus Infections and Treatments
Lymphoma Diagnosis and Treatment
Cancer-related molecular mechanisms research
Jiao Song
University of Arkansas for Medical Sciences
Herpesvirus Infections and Treatments
Cancer-related molecular mechanisms research
Host-Pathogen Interactions
Kennith Swafford
University of Arkansas for Medical Sciences
Lymphoma Diagnosis and Treatment
Cancer-related molecular mechanisms research
Genomics and Chromatin Dynamics
Samantha Kendrick
University of Arkansas for Medical Sciences
Herpesvirus Infections and Treatments
Lymphoma Diagnosis and Treatment
Cancer-related molecular mechanisms research
Reine U Protacio
University of Arkansas for Medical Sciences
CRISPR and Genetic Engineering
Host-Pathogen Interactions
Genomics and Chromatin Dynamics