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Current · Arkansas
Last published
2026
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OpenAlex · ORCID
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2026-10-06
V
Vasily N. Dobrovolsky
This is a likely match — the affiliation was inferred from OpenAlex, ORCID, and web sources but has not been fully confirmed. Treat with appropriate caution.
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Also affiliated: Russian Academy of Sciences (1993–1995); United States Food and Drug Administration (1999–2026); Institute of Bioorganic Chemistry (1993–1995)
29 h-index
102 pubs
2,577 cited
Research Areas
Biomedical Subjects
Links
Biography and Research Information
OverviewAI-generated summary
Vasily N. Dobrovolsky's research focuses on genotoxicity assessment, particularly the development and application of in vivo gene mutation assays. He has investigated the Pig-A gene mutation assay, utilizing flow cytometry to detect mutant peripheral red blood cells and spleen T-cells in rats. This work includes studies on the accumulation and persistence of Pig-A mutant cells following exposure to chemical mutagens, such as N-ethyl-N-nitrosourea.
His publications also include research on the genotoxicity of specific compounds, such as malachite green, leucomalachite green, acrylamide, and glycidamide, primarily using Big Blue mice and rats. Dobrovolsky has collaborated extensively with researchers at the National Center for Toxicological Research, including Page B. McKinzie, Javier R. Revollo, Jaime A. Miranda, and Robert H. Heflich, resulting in multiple shared publications. His work is recognized by a high h-index of 29 and over 2,500 citations across more than 100 publications.
Metrics
- h-index: 29
- Publications: 102
- Citations: 2,577
Selected Publications
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Using error-corrected sequencing for evaluating mutagenicity of molnupiravir in humans (2026)
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Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes (2025)
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Application of error-corrected sequencing technologies for in vivo regulatory mutagenicity assessment (2025)
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Erythrocyte PIG‐A mutant frequencies in cancer patients receiving cisplatin (2024)
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Whole-genome high-fidelity sequencing: A novel approach to detecting and characterization of mutagenicity in vivo (2023)
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Unbiased whole genome detection of ultrarare off‐target mutations in genome‐edited cell populations by HiFi sequencing (2023)
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Establishment of neural stem cells from fetal monkey brain for neurotoxicity testing (2023)
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Evaluation of the mutagenic effects of Molnupiravir and N4 ‐hydroxycytidine in bacterial and mammalian cells by HiFi sequencing (2022)
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Genome‐wide detection of ultralow‐frequency substitution mutations in cultures of mouse lymphoma L5178Y cells and Caenorhabditis elegans worms by PacBio sequencing (2022)
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PacBio sequencing detects genome‐wide ultra‐low‐frequency substitution mutations resulting from exposure to chemical mutagens (2021)
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Mutational signatures in T‐lymphocytes of rats treated with N ‐propyl‐ N‐nitrosourea and procarbazine (2021)
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Pig‐a gene mutations in bone marrow granulocytes of procarbazine‐treated F344 rats (2021)
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Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice (2020)
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Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes (2020)
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Molecular analysis of GPI-anchor biosynthesis pathway genes in rat strains used for the Pig-a gene mutation assay (2020)
Collaboration Network
Top Collaborators
Robert H. Heflich
National Center for Toxicological Research
58 shared publications
In database
- The in vivo Pig-a assay: A report of the International Workshop On Genotoxicity Testing (IWGT) Workgroup
- Development of an in vivo gene mutation assay using the endogenous Pig‐A gene: I. Flow cytometric detection of CD59‐negative peripheral red blood cells and CD48‐negative spleen T‐cells from the rat
- The in vivo pig‐a gene mutation assay, a potential tool for regulatory safety assessment
- Genotoxicity of malachite green and leucomalachite green in female Big Blue B6C3F1 mice
- Accumulation and persistence of Pig-A mutant peripheral red blood cells following treatment of rats with single and split doses of N-ethyl-N-nitrosourea
Showing 5 of 58 shared publications
Joseph G. Shaddock
National Center for Toxicological Research
26 shared publications
In database
- Genotoxicity of malachite green and leucomalachite green in female Big Blue B6C3F1 mice
- Genotoxicity of furan in Big Blue rats
- Report on stage III Pig‐a mutation assays using N‐ethyl‐N‐nitrosourea – comparison with other in vivo genotoxicity endpoints
- Report on stage IIIPig‐amutation assays using benzo[a]pyrene
- Evaluation of Macaca mulatta as a model for genotoxicity studies
Showing 5 of 26 shared publications
Javier R. Revollo
National Center for Toxicological Research
23 shared publications
In database
- Confirmation of Pig-a mutation in flow cytometry-identified CD48-deficient T-lymphocytes from F344 rats
- Evaluation of the mutagenic effects of Molnupiravir and N4 ‐hydroxycytidine in bacterial and mammalian cells by HiFi sequencing
- CD48‐deficient T‐lymphocytes from DMBA‐treated rats have de novo mutations in the endogenous Pig‐a gene
- Spectrum of Pig-a mutations in T lymphocytes of rats treated with procarbazine
- Glycosylphosphatidylinositol (GPI) anchored protein deficiency serves as a reliable reporter of Pig‐a gene Mutation: Support from an in vitro assay based on L5178Y/Tk+/− cells and the CD90.2 antigen
Showing 5 of 23 shared publications
Mason G. Pearce
National Center for Toxicological Research
22 shared publications
In database
- In vivo genotoxicity assessment of acrylamide and glycidyl methacrylate
- Report on stage III Pig‐a mutation assays using N‐ethyl‐N‐nitrosourea – comparison with other in vivo genotoxicity endpoints
- Report on stage IIIPig‐amutation assays using benzo[a]pyrene
- Confirmation of Pig-a mutation in flow cytometry-identified CD48-deficient T-lymphocytes from F344 rats
- Sensitivity of the Pig-a assay for detecting gene mutation in rats exposed acutely to strong clastogens
Showing 5 of 22 shared publications
Roberta A. Mittelstaedt
National Center for Toxicological Research
17 shared publications
In database
- Genotoxicity of malachite green and leucomalachite green in female Big Blue B6C3F1 mice
- Development of an in vivo gene mutation assay using the endogenous Pig‐A gene: II. Selection of Pig‐A mutant rat spleen T‐cells with proaerolysin and sequencing Pig‐A cDNA from the mutants
- Genotoxicity of furan in Big Blue rats
- Confirmation of Pig-a mutation in flow cytometry-identified CD48-deficient T-lymphocytes from F344 rats
- Evaluation of Macaca mulatta as a model for genotoxicity studies
Showing 5 of 17 shared publications
Page B. McKinzie
National Center for Toxicological Research
15 shared publications
In database
- Evaluation of the mutagenic effects of Molnupiravir and N4 ‐hydroxycytidine in bacterial and mammalian cells by HiFi sequencing
- Spectrum of Pig-a mutations in T lymphocytes of rats treated with procarbazine
- Glycosylphosphatidylinositol (GPI) anchored protein deficiency serves as a reliable reporter of Pig‐a gene Mutation: Support from an in vitro assay based on L5178Y/Tk+/− cells and the CD90.2 antigen
- Spectrum of benzo[ a ]pyrene-induced mutations in the Pig-a gene of L5178Y Tk +/− cells identified with next generation sequencing
- Establishing a novel Pig‐a gene mutation assay in L5178YTk+/− mouse lymphoma cells
Showing 5 of 15 shared publications
Daishiro Miura
Teijin (Japan) (JP)
12 shared publications
- Development of an in vivo gene mutation assay using the endogenous Pig‐A gene: I. Flow cytometric detection of CD59‐negative peripheral red blood cells and CD48‐negative spleen T‐cells from the rat
- The in vivo pig‐a gene mutation assay, a potential tool for regulatory safety assessment
- Accumulation and persistence of Pig-A mutant peripheral red blood cells following treatment of rats with single and split doses of N-ethyl-N-nitrosourea
- Development of an in vivo gene mutation assay using the endogenous Pig‐A gene: II. Selection of Pig‐A mutant rat spleen T‐cells with proaerolysin and sequencing Pig‐A cDNA from the mutants
- Manifestation of Pig-a mutant bone marrow erythroids and peripheral blood erythrocytes in mice treated with N-ethyl-N-nitrosourea: Direct sequencing of Pig-a cDNA from bone marrow cells negative for GPI-anchored protein expression
Showing 5 of 12 shared publications
Suzanne M. Morris
National Center for Toxicological Research (US)
10 shared publications
- Genotoxicity of malachite green and leucomalachite green in female Big Blue B6C3F1 mice
- Evaluation of Macaca mulatta as a model for genotoxicity studies
- Pharmacokinetics, dose‐range, and mutagenicity studies of methylphenidate hydrochloride in B6C3F1 mice
- The genetic toxicology of methylphenidate hydrochloride in non-human primates
- p53‐competent cells and p53‐deficient cells display different susceptibility to oxygen functionalized graphene cytotoxicity and genotoxicity
Showing 5 of 10 shared publications
Azra Dad
National Center for Toxicological Research
10 shared publications
In database
- Spectrum of benzo[ a ]pyrene-induced mutations in the Pig-a gene of L5178Y Tk +/− cells identified with next generation sequencing
- Establishing a novel Pig‐a gene mutation assay in L5178YTk+/− mouse lymphoma cells
- Genome-wide mutation detection by interclonal genetic variation
- Evaluation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) mutagenicity using in vitro and in vivo Pig-a assays
- Analysis of mutation in the rat Pig‐a assay: I) studies with bone marrow erythroid cells
Showing 5 of 10 shared publications
Javed A. Bhalli
Covance (United States)
8 shared publications
In database
- Report on stage III Pig‐a mutation assays using N‐ethyl‐N‐nitrosourea – comparison with other in vivo genotoxicity endpoints
- Report on stage IIIPig‐amutation assays using benzo[a]pyrene
- Sensitivity of the Pig-a assay for detecting gene mutation in rats exposed acutely to strong clastogens
- Manifestation and persistence of Pig‐a mutant red blood cells in C57BL/6 mice following single and split doses of N‐ethyl‐N‐nitrosourea
- Evaluating the weak in vivo micronucleus response of a genotoxic carcinogen, Aristolochic acids
Showing 5 of 8 shared publications
Dayton M. Petibone
National Center for Toxicological Research
7 shared publications
In database
- Confirmation of Pig-a mutation in flow cytometry-identified CD48-deficient T-lymphocytes from F344 rats
- The genetic toxicology of methylphenidate hydrochloride in non-human primates
- p53‐competent cells and p53‐deficient cells display different susceptibility to oxygen functionalized graphene cytotoxicity and genotoxicity
- Whole genome and normalized mRNA sequencing reveal genetic status of TK6, WTK1, and NH32 human B-lymphoblastoid cell lines
- In Vivo Rat T-Lymphocyte Pig-a Assay: Detection and Expansion of Cells Deficient in the GPI-Anchored CD48 Surface Marker for Analysis of Mutation in the Endogenous Pig-a Gene
Showing 5 of 7 shared publications
Takafumi Kimoto
Teijin (Japan) (JP)
7 shared publications
- The in vivo Pig-a assay: A report of the International Workshop On Genotoxicity Testing (IWGT) Workgroup
- Accumulation and persistence of Pig-A mutant peripheral red blood cells following treatment of rats with single and split doses of N-ethyl-N-nitrosourea
- Manifestation of Pig-a mutant bone marrow erythroids and peripheral blood erythrocytes in mice treated with N-ethyl-N-nitrosourea: Direct sequencing of Pig-a cDNA from bone marrow cells negative for GPI-anchored protein expression
- Further development of the rat Pig‐a mutation assay: Measuring rat Pig‐a mutant bone marrow erythroids and a high throughput assay for mutant peripheral blood reticulocytes
- Flow cytometric detection of Pig‐A mutant red blood cells using an erythroid‐specific antibody: Application of the method for evaluating the in vivo genotoxicity of methylphenidate in adolescent rats
Showing 5 of 7 shared publications
Mugimane G. Manjanatha
National Center for Toxicological Research
6 shared publications
In database
- Flow cytometric detection of Pig‐A mutant red blood cells using an erythroid‐specific antibody: Application of the method for evaluating the in vivo genotoxicity of methylphenidate in adolescent rats
- Evaluation of Macaca mulatta as a model for genotoxicity studies
- Pharmacokinetics, dose‐range, and mutagenicity studies of methylphenidate hydrochloride in B6C3F1 mice
- The genetic toxicology of methylphenidate hydrochloride in non-human primates
- Evaluation of mutagenic mode of action in Big Blue mice fed methylphenidate for 24 weeks
Showing 5 of 6 shared publications
Yoshinori Kasahara
Teijin (Japan) (JP)
6 shared publications
- Development of an in vivo gene mutation assay using the endogenous Pig‐A gene: I. Flow cytometric detection of CD59‐negative peripheral red blood cells and CD48‐negative spleen T‐cells from the rat
- Accumulation and persistence of Pig-A mutant peripheral red blood cells following treatment of rats with single and split doses of N-ethyl-N-nitrosourea
- Development of an in vivo gene mutation assay using the endogenous Pig‐A gene: II. Selection of Pig‐A mutant rat spleen T‐cells with proaerolysin and sequencing Pig‐A cDNA from the mutants
- Manifestation of Pig-a mutant bone marrow erythroids and peripheral blood erythrocytes in mice treated with N-ethyl-N-nitrosourea: Direct sequencing of Pig-a cDNA from bone marrow cells negative for GPI-anchored protein expression
- Further development of the rat Pig‐a mutation assay: Measuring rat Pig‐a mutant bone marrow erythroids and a high throughput assay for mutant peripheral blood reticulocytes
Showing 5 of 6 shared publications
Timothy W. Robison
United States Food and Drug Administration (US)
6 shared publications
- Monitoring humans for somatic mutation in the endogenous pig‐a gene using red blood cells
- Analysis of mutation in the rat Pig‐a assay: I) studies with bone marrow erythroid cells
- Pig-a mutations in bone marrow erythroblasts of rats treated with 7,12-dimethyl-benz[a]anthracene
- CD59 ‐deficient bone marrow erythroid cells from rats treated with procarbazine and propyl‐nitrosourea have mutations in thePig‐agene
- Mutational signatures in T‐lymphocytes of rats treated with N ‐propyl‐ N‐nitrosourea and procarbazine
Showing 5 of 6 shared publications
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